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¿µ³²ÀÇ´ëÇмúÁö Vol.24_No.2 Suppl. P.S365-372, Dec. 2007

Original Article

Interleukin-1À¸·Î À¯µµµÈ ÈòÁã ±Þ¼ºÆó¼Õ»ó¿¡¼­ Aerosolized Vitamin-EÀÇ Àüóġ È¿°ú

Effect of on Aerosolized Vitamin E Pretreatment on Interleukin-1 Induced Acute Lung Injury in Rats

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Tel: (053) 620-3830, Fax: (053) 654-8386
E-mail: jhchn@med.yu.ac.kr

December 30, 2007

Abstract

Background£ºInterleukin-1 (IL-1) and neutrophil appear to contribute to the pathogenesis of acute respiratory distress syndrome (ARDS). Reactive oxygen species, as well as elastase released from activated neutrophil, are thought to play pivotal roles in the experimental models of acute lung leak. This study investigated whether aerosolized vitamin E can attenuate acute lung injury induced by IL-1 in rats.
Materials and Methods£ºWe intratracheally instilled either saline or IL-1 with and without pretreatment with aerosolized vitamin E in rats. After 5 hours of intratracheal instillation, lung lavage neutrophils, lung lavage protein concentration, lung myeloperoxidase(MPO) activity and lung wet weight to dry weight ratio(WW/DW) were measured in rat.
Results£ºIn rats given IL-1 intratracheally, lung lavage neutrophils, lung lavage protein concentration, lung MPO activity and WW/DW were higher. Pretreatment with aerosolized vitamin E decreased lung lavage neutrophils, lung MPO activity and WW/DW in rats given IL-1 intratracheally.
Conclusion£ºThese results suggest that direct pulmonary supplement of vitamin E decreases lung inflammation and leak in rats given IL-1 intratracheally.

Key Words: Acute lung injury, Interleukin-1, Aerosolized vitamin E

References

1. Bernard GR, Artigas A, Brigham KL, Carlet J, Falke K, Hudson L, et al. The American- European Consensus Conference on ARDS. Definitions, mechanisms, relevant outcomes, and clinical trial coordination. Am J Respir Crit Care Med 1994 Mar;149(3 pt 1):818-24.

2. Ashbaugh DG, Bigelow DB, Petty TL, Levine BE. Acute respiratory distress in adult. Lancet 1967 Aug 12;2(7511):319-23.

3. Àå ÁØ: ¼ºÀμº È£Èí°ï¶õ ÁõÈıºÀÇ º´Å»ý¸®. ´ëÇѳ»°úÇÐȸÁö 1994;46(suppl II):198-205.

4. Repine JE. Scientific perspectives on adult respiratory distress syndrome. Lancet 1992 Feb 22;339(8791):466-9.

5. Dillard CJ, Litor RE, Savin WM, Dumelin E, Tappel AL. Effects of exercise, vitamin E and ozone on pulmonary fuction and lipid peroxi- dation. J Appl Physiol 1978 Dec 25;45(6): 927-32.

6. Yoshikawa T, Furukawa Y, Murakami M, Vatanabe K, Kondo M. Effect of vitamin E on endotoxin-induced disseminated intravascular coagulation in rats. Thromb Haemost 1982 Oct 29;48(2):235-7.

7. Chow CK. Vitamin E and oxidative stress. Free Radic Biol Med 1991;11(2):215-32.

8. Hybertson BM, Leff JA, Beehler CJ, Barry PC, Repine JE. Effect of vitamin E deficiency and supercritical fluid aerosolized vitamin E supplementation on interleukin-1 induced oxidative lung injury in rats. Free Radic Biol Med 1995 Mar;18(3):537-42.

9. Hybertson BM, Kitlowski RP, Jepson EK, Repine JE. Supercritical fluid-aerosloized vitamin E pretreatment decreases leak in isolated oxidant-perfused rat lungs. J Appl Physiol 1998 Jan;84(1):263-8.

10. Jacobs RF, Tabor DR, Burks AW, Campbell GD. Elevated interleukin-l release by human alveolar macrophages during the adult respiratory distress syndrome. Am Rev Resp Dis 1989 Dec;140(6):1686-92.

11. Guidot DM, Stevens EE, Repine MJ, Lucca- Broco AE, Repine JE. Intratracheal but not intravascular interleukin-l causes acute edematous injury in isolated neutrophil-perfused rat lungs through an oxygen radical mediated mechanism. J Lab Clin Med 1994 Apr;123(4): 605-9.

12. Leff JA, Baer JW, Bodman ME, Kirkman JM, Shanley PF, Patton LM, et al. Interleukin- 1-induced lung neutrophil accumulation and oxygen metabolite-mediated lung leak in rats. Am J Physiol 1994 Jan;266(1 pt 1):L2-L8.

13. Koh Y. Hybertson BM, Jepson EK, Cho OJ, Repine JE. Cytokine-induced neutrophil chemoattractant is necessary for interleukin-l- induced lung leak in rats. J Appl Physiol 1995 Aug;79(2):472-8.

14. Hybertson BM, Jepson EK, Clarke JH, Spelts RJ, Repine JE. Intrleukin-1 stimulates rapid release of cytokine-induced neutrophil chemoat- tractant (CINC) in rat lungs. Inflammation 1996 Oct;20(5):471-83.

15. Hybertson BM, Bursten SL, Leff JA, Lee YM, Jepson EK, Dewitt CR, et al. Lisofylline prevents leak, but not neutrophil accumulation, in lungs of rats given IL-1 intratracheally. J Appl Physiol 1997 Jan;82(1):226-32.

16. Lee YM, Hybertson BM, Terada LS, Repine AJ, Cho HG, Repine JE. Mepacrine decreases lung leak in rats given interleukin-1 intratracheally. Am J Respir Crit Care Med 1997 Mar;155(5): 1624-8.

17. Jung JH, Mun YC, Park HJ, Shin KC, Lee KH. Effect of neutrophil elastase inhibitor, ICI 200,355 on interleukin-1 induced acute lung injury. yeungnam Univ J Med 2002;19(1):55-62.

18. ¹Ú¼º¼ö. ¼ºÀμº È£Èí°ï¶õ ÁõÈıºÀÇ Ä¡·á. ´ëÇѳ»°úÇÐȸÁö 1994;46(suppl II):206-215.

19. Ehrenkranz RA, Ablow RC, Warshaw JB. Prevention of bronchopulmonary dysplasia with vitamin E administration during the acute stages of respiratory distress syndrome. J Pediatr 1979 Nov;95(5 pt 2):873-8.

20. Chabot F, Mitchell JA, Gutteridge JMC, Evans TW. Reactive oxygen species in acute lung injury. Eur Respir J 1998 Mar;11(3):745-57.

21. Suzuki Y, Tanigaki T, Heimer D, Wang WZ, Ross WG, Sussman HH, et al.. Polyethylene glycol-conjugated superoxide dismutase attenuates septic lung injury in guinea pigs. Am Res Respir Dis 1992 Feb;145(2 pt 1):388- 93.

22. Amari T, Kubo K, Kobayashi T, Sekiguchi M. Effect of recombinant human superoxide dismutase on tumor necrosis factor-induced lung injury in awake sheep. J Appl physiol 1993 Jun;74(6):2641-8.

23. Boissinot M, Kuhn LA, Lee P, Fisher CL, Wang Y, Hallewell RA, et al. Rational design and expression of a heparin-targeted human superoxide dismutase. Biochem Biophys Res Comm 1993 Jan 15;190(1):250-6.

24. Turrens JF, Crapo JD, Freeman BA. Protection against oxygen toxicity by intravenous injection of liposome-entrapped catalase and superoxide dismutase. J Clin Invest 1984 Jan;73(1):87-95.

25. McCord JM. Superoxide radical: controversies, contradictions, and paradoxes. Proc Soc Exp Biol Med 1995 Jan;209(2):112-7.

26. McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 1985 Jan 17;312(3):159-63.

27. Jepsen S, Herlevsen P, Knudsen P, Bud MI, Klausen NO. Antioxidant treatment with N-acetylcysteine during adult respiratory distress syndrome: a prospective, randomized, placebo-controlled study. Crit Care Med 1992 Jul;20(7):918-23.

28. Suter PM, Domenighetti G, Schaller MD, Laverriere MC, Ritz R, Perret C. N-acetylcy- steine enhances recovery from acute lung injury in man. A randomized, double-blind, placebo-controlled clinical study. Chest 1994 Jan;105(1):190-4.

29. Seeger W, Ziegler A, Wolf HR. Serum ¥á-tocopherol levels after high-dose enteral vitamin E administration in patients with acute respiratory failure. Intensive Care Med 1987;13(6):395-400.

30. Peake IR, Windmueller HG, Bieri JG. A comparison of the intestinal absorption, lymph and plasma transport, and tissue uptake of ¥á- and r-tocopherols in the rats. Biochem Biophys Acta 1972 Apr 18;260(4):679-88.

31. Suntres ZE, Hepworth SR, Shek PN. Pulmonary uptake of liposome-associated alpha-tocopherol following intratracheal instillation in rats. J Pharm Pharmacol 1993 Jan;45(6):514-20.

32. Toth KM, Harlan JM, Berger EM, Parker NB, Repine JE. Dimethylthiourea prevents hydrogen peroxide and neutrophil mediated damage to lung endothelial cells in vitro and disappears in the process. Free Radic Biol Med 1989;6 (5):457-66.

33. Rubanyi GM, Ho EH, Cantor EH, Lumma WC, Botelho LH. Cytoprotective function of nitric oxide: inactivation of superoxide radicals produced by human leukocytes. Biochem Biophys Res Commun 1991 Dec 31;181(3):1392-7.

34. Galili Y, Ben-Abraham R, Weinbroum A, Marmur S, Laina A, Volman Y, et al. Methylene blue prevents pulmonary injury after intestinal ischemia-reperfusion. J Trauma 1998 Aug;45 (2):222-5.

35. Nakayama M, Hasegawa N, Oka Y, Lutzke B, McCall JM, Raffin TA. Effects of the lazaroid, tirilazad mesylate on sepsis-induced acute lung injury in minipigs. Crit Care Med 1998 Mar;26(3):538-47.