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Ǵм Yeungnam Univ J Med 2018;35(1):63-69


Reliability and validity of free software for the analysis of locomotor activity in mice

Yoo Rha Hong1, Eunsoo Moon2,3
1Department of Pediatrics, College of Medicine, Kosin University; 2Department of Psychiatry, Pusan National University Hospital;
3Department of Psychiatry, Pusan National University School of Medicine, Busan, Korea
Corresponding Author: Eunsoo Moon, Department of Psychiatry, Pusan National University Hospital, Pusan National University School of Medicine, 179, Gudeok-ro, Seo-gu, Busan 49241, Korea
Tel: +82-51-240-7303, Fax: +82-51-248-3648
E-mail: esmun@hanmail.net

Received: April 30, 2018, Revised: May 23, 2018 Accepted: May 25, 2018


Background: Kinovea software that tracking semi-automatically the motion in video screen has been used to study motion-related tasks in several studies. However, the validation of this software in open field test to assess locomotor activity have not been studied yet. Therefore, this study aimed to examine the reliability and validity of this software in analyzing locomotor activities. Methods: Thirty male Institute Cancer Research mice were subjected in this study. The results examined by this software and the classical method were compared. Test-retest reliability and inter-rater reliability were analyzed with Pearson's correlation coefficient and intraclass correlation coefficient (ICC). The validity of this software was analyzed with Pearson's correlation coefficient. Results: This software showed good test-retest reliability (ICC=0.997, 95% confidence interval [CI]=0.975- 0.994, p<0.001). This software also showed good inter-rater reliability (ICC=0.987, 95% CI=0.973-0.994, p<0.001). Furthermore, in three analyses for the validity of this software, there were significant correlations between two methods (Pearson's correlation coefficient=0.928-0.972, p<0.001). In addition, this software showed good reliability and validity in the analysis locomotor activity according to time interval. Conclusion: This study showed that this software in analyzing drug-induced locomotor activity has good reliability and validity. This software can be effectively used in animal study using the analysis of locomotor activity.

Key Words: Keywords: Locomotor activity; Video-tracking software; Reliability; Validity; Open field test