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Ǵм Vol.24_No.2 Suppl. P.S606-616, Dec. 2007

Original Article

Apoptosis Induced by 4-HPR on Human Stomach Adenocarcinoma Cell Line SNU1*

Hyou Youn Kim, Sang Woon Kim, Seong Yong Kim
Department of Biochemistry and Molecular Biology,
Department of General Surgery, College of Medicine, Yeungnam University, Daegu, Korea
åڣ輺, 뱸 317-1, б ǰ ȭ?ڻб
Tel: (053) 620-4343, Fax: (053) 654-6651
E-mail: seongyong@med.yu.ac.kr

December 30, 2007

Abstract

PurposeRetinoids derived from vitamin A have diverse effects on development, morphogenesis, and homeostasis. They also have effects for prevention and treatment of cancers. In this study, the effect of N-(4-hydroxyphenyl) retinamide (4-HPR) on growth and/or proliferation of human gastric adenocarcinoma cell line SNU1 was investigated.
Materials and MethodsThe cytotoxic effect of 4-HPR was assessed by MTT assay. The apoptosis induced by 4-HPR was analyzed with cytoplasmic DNA Fragmentation, flow cytometry, and Western blot.
Results4-HPR induced cell death of SNU1. The cytoplasmic DNA fragmentation was increased time dependently after treatment of 4-HPR and the cells in the sub-G0/G1 fraction of flow cytometric analysis were also increased time dependently after treatment of 4-HPR. The cleavage of caspase 3 and PARP were detected after treatment of 4-HPR to SNU1. The phosphorylations of Raf, ERK and AKT were induced by 4-HPR but after pre-treatment of MAPK inhibitor (PD98059) or PI-3 kinase inhibitor (LY294002) the 4-HPR-induced cytoplasmic DNA fragmentation, the cells in the sub-G0/G1 fraction fraction of flow cytometric analysis, and cleavage of caspase 3 and PARP were diminished in SNU1 cells.
ConclusionThe results show that 4-HPR induces apoptosis of SNU1 and this 4-HPR- induced apoptosis may be mediated through ERK1/2 and PI3 kinase signaling pathways in SNU1.

* 2005⵵ Ͼǰ Ͽ ̷.

Key Words: 4-HPR, SNU1, apoptosis.

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