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영남의대학술지 Vol.24_No.2 Suppl. P.S456-462, Dec. 2007
Original Article
Comparison of REP13E12 PCR with Amplicor MTB for the Detection of Mycobacterium tuberculosis in Respiratory Specimens
Tae-Yoon Lee
Department of Microbiology, College of Medicine, Yeungnam University, Daegu, Korea
책임저자：이태윤, 대구광역시 남구 대명5동 317-1, 영남대학교 의과대학 미생물학교실
Tel: (053) 620-4362, Fax: (053) 653-6628
E-mail: doxr7p@med.yu.ac.kr
December 30, 2007
Abstract
Backgrounds：In recent years, the incidence of tuberculosis has increased mainly in high-risk populations. Classical laboratory diagnostic methods for tuberculosis have low sensitivity and time consuming procedures. Thus, it is important to identify the presence of Mycobacterium tuberculosis in the clinical specimens earlier than the culture results for the decision to initiate anti-tuberculosis therapy. Lee et al. reported a species-specific repeated sequence from a Korean M. tuberculosis isolate, which was later proved to be a part of REP13E12 repetitive sequence. Materials and Methods：In this study, we compared the acid-fast staining, culture, Amplicor MTB (Roche), and REP13E12 PCR for detection of M. tuberculosis using 88 clinical samples. The sensitivity, specificity, positive and negative predictive values were compared. Results：REP13E12 PCR showed equivalent score to Amplicor MTB. Both PCR-based methods showed better score than conventional stain and culture methods. Conclusion：This result suggested that REP13E12 PCR is helpful for the rapid detection of the M. tuberculosis from clinical specimens.
Key Words: Mycobacterium tuberculosis, Detection, REP13E12-PCR
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