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영남의대학술지 Vol.24_No.2 Suppl. P.S449-455, Dec. 2007

Original Article

영남대학교병원에서 동정된 비예기 항체의 빈도 및 분포: 2002년 1월에서 2006년 12월까지 최근 5년간의 경험

The Frequency and Distribution of Unexpected Antibodies at Yeungnam University Hospital: Recent Five Years Experience from January, 2002 to December, 2006

조희순, 이채훈, 김경동
영남대학교 의과대학 진단검사의학교실
책임저자:조희순, 대구광역시 남구 대명동 317-1, 영남대학교 의과대학 진단검사의학교실
Tel: (053) 620-3633, Fax: (053) 653-7774
E-mail: chscp@med.yu.ac.kr

December 30, 2007

Abstract

Background:Unexpected antibodies can cause hemolytic transfusion reaction or decrease the survival of transfused red blood cells. Type and screen is recommended for efficient transfusion, so we perform antibody screening test for pre-transfusion test. In this study, we investigated the frequency and distribution of unexpected antibodies at Yeungnam University Hospital during recent 5 years.
Methods:We analyzed the results of 28,944 antibody screening test that were performed for preparation of packed red cells from 2002 to 2006. Screening and identification of unexpected antibodies were carried out using a column agglutination method with the DiaMed ID system.
Results:Unexpected antibodies were detected from 234 samples (0.84%) out of 28,944 samples. After exclusion of samples from same patients, 185 patients had unexpected antibodies. Identification of unexpected antibodies were performed from 108 patients. Antibody that was detected most frequently was anti-E (36.1%), followed by anti-C and anti-Lea (6.5%), anti-c (4.6%), anti-D (3.7%), anti-Jkb (3.0%), anti-Jka, anti-S, anti-Xga (1.9%), anti-e, anti-Fya, anti-M, anti-Jsa and anti-Lub (0.9%). 15 patients (13.9%) had autoantibodies and 20 cases (18.5%) could not be identified by LISS/Coombs method.
Conclusion:The frequency of unexpected antibodies at our hospital was 0.84%, and the most identified unexpected antibodies were clinically significant. Therefore, antibody screening and identification tests are critical step in pre-transfusion test.

Key Words: Unexpected antibody, Type and screen, Column agglutination method

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